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Nova® Bis-Tris Gel
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  • Nova® Bis-Tris Gels are precast polyacrylamide gels designed to give optimal separation of small to medium-sized proteins. Nova® Bis-Tris gels have a neutral pH environment that minimizes protein modifications resulting in sharper bands without typical gel "smiling" (Figure 1). Nova® Bis-Tris gels can be used for sequencing, mass spectrometry, and any other application in which protein integrity is crucial.

    Nova® Bis-Tris Gels are designed to allow:
    • Separation of a wide range of molecular weight proteins
    • Preservation of protein sample integrity
    • Faster run times (completed in as little as 35 minutes)
    • A longer shelf life (16 months* at room temperature without loss of performance)
    • Using gels from another company? Use our product selection guide to find the Life equivalent!
    *from date of manufacture
     
    The Nova® Bis-Tris discontinuous buffer system involves three ions:
    • Chloride (–) from the gel buffer and serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are Bis-Tris+ and Cl– (pH 6.4).
    • MES or MOPS (–) serve as the trailing ion in the running buffer. The running buffer ions are Tris+, MOPS-/MES–, and dodecylsulfate- (pH 7.3–7.7).
    • Nova® Bis-Tris (+) is the common ion present in the gel buffer and running buffer. The combination of the lower pH gel buffer (pH 6.4) and the running buffer (pH 7.3–7.7) results in a significantly lower operating pH of 7 during electrophoresis
  • Gel dimensions
     (W x L x thickness), cm
    8x 8x 0.1
    Cassette dimensions
     (W x L x thickness), cm
    10x 10 x 0.6
    Cassette material
    Styrene copolymer
    Comb material
    Polycarbonate
    Gel storage conditions
    Store flat at 2–8°C; do not freeze
    Shelf life at recommended temperature*
    12 months
    Recommended sample buffer
    (Laemmli, dilute 1:1 with sample)
    62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
    Recommended running buffer
    (Tris/glycine/SDS)
    25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
    Gel format
    Mini
    * From date of manufacture.
     

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